Coding

Part:BBa_K3402010

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-01)


SBLE

SBLE will express an enzyme in Starmerella bombicola which plays a catalytic role in the production pathway of sophorolipid. This enzyme can change the configuration of sophorolipid.

Usage and Biology

The gene of a new enzyme was found in the extracellular protein of S. bombicola. In the strains that lack the enzyme, there will not be any lactone-type sophorolipids. SBLE catalyzed an unusual reaction to convert acid-type sophorolipids into lactone-type sophorolipids in aqueous environment. However, it is still unclear how SBLE catalyze intermolecular lactonization in aqueous environment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 553


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Parameters
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